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Characterization of a recombinant Newcastle disease virus vaccine strain
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Sun-Hee | - |
dc.contributor.author | Kwon, Hyuk-Joon | - |
dc.contributor.author | Kim, Tae-Eun | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.contributor.author | Yoo, Han Sang | - |
dc.contributor.author | Park, Man-Hoon | - |
dc.contributor.author | Park, Yong Ho | - |
dc.contributor.author | Kim, Sun-Joong | - |
dc.date.accessioned | 2009-08-31T23:58:04Z | - |
dc.date.available | 2009-08-31T23:58:04Z | - |
dc.date.issued | 2008-09-03 | - |
dc.identifier.citation | Clin. Vaccine Immunol. 15:1572-1579 | en |
dc.identifier.issn | 1556-6811 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8314 | - |
dc.description.abstract | A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase
(HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from 112RRQKR116 to 112GRQAR116, and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids by single point mutation was inserted at codon 115. In addition a six-nucleotide sequence was inserted into the intergenic region between matrix protein and F genes for attenuation without breaking the rule-of-six. The HN protein length was increased from 571 to 577 as a marker. Serological tests revealed that the antigenicity of KBNP-C4152R2L was similar to that of KBNP-4152 but distinct from that of the La Sota strain. KBNPC4152R2L was avirulent (intracerebral pathogenicity index, 0.0; mean death time, >168 h) and stable in pathogenicity through in vivo passages. The killed oil emulsion of and live KBNP-C4152R2L were completely protective against mortality and egg drop caused by virulent strains, and KBNP-C4152R2L was applicable to in ovo vaccination. Therefore, KBNP-C4152R2L is a promising vaccine strain and viral vector in terms of antigenicity, productivity, safety, and pathogenic stability. | en |
dc.description.sponsorship | This study was supported by the Regional Industrial Technology
Development Program of the Ministry of Commerce, Industry, and Energy, Korea. TV vector was kindly provided by Andrew Ball (University of Alabama, Birmingham). | en |
dc.language.iso | en | en |
dc.publisher | American Society for Microbiology | en |
dc.title | Characterization of a recombinant Newcastle disease virus vaccine strain | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 조선희 | - |
dc.contributor.AlternativeAuthor | 권혁준 | - |
dc.contributor.AlternativeAuthor | 김태은 | - |
dc.contributor.AlternativeAuthor | 김재홍 | - |
dc.contributor.AlternativeAuthor | 유한상 | - |
dc.contributor.AlternativeAuthor | 박만훈 | - |
dc.contributor.AlternativeAuthor | 박용호 | - |
dc.contributor.AlternativeAuthor | 김선중 | - |
dc.identifier.doi | 10.1128/CVI.00156-08 | - |
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