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Potentiated therapeutic angiogenesis by primed human mesenchymal stem cells in a mouse model of hindlimb ischemia

Cited 28 time in Web of Science Cited 32 time in Scopus
Authors
Lee, Eun Ju; Park, Hwan-Woo; Jeon, Hyo-Jin; Kim, Hyo-Soo; Chang, Mi-Sook
Issue Date
2013-05
Publisher
Future Science Group
Citation
Regenerative Medicine Vol.8 No.3, pp. 283-293
Keywords
의약학angiogenesisischemiamesenchymal stem cellsparacrine effecttransplantation
Abstract
Background: Human bone marrow-derived mesenchymal stem cells (hMSCs) are advantageous for cell-based therapy to treat ischemic diseases owing to their capacity to secrete various paracrine factors with potent angiogenic activity. Materials methods: In this study, we describe a method to increase secreted levels of VEGF and HGF from hMSCs without genetic modification. Results: We demonstrated that transplantation of primed hMSCs into ischemic limbs led to significantly greater improvements in tissue perfusion and limb salvage by increasing capillary formation compared with nonprimed hMSCs. The primed hMSCs also exhibited greater survival in vivo and secreted human VEGF and HGF in the ischemic tissue, supporting enhanced angiogenesis and cell survival. Conclusion: These findings indicate that priming hMSCs via methods described in this study enhances secretion of critical proangiogenic factors resulting in an enhanced therapeutic effect of cells for the treatment of ischemic diseases.
ISSN
1746-0751
Language
English
URI
https://hdl.handle.net/10371/83482
DOI
https://doi.org/10.2217/rme.13.17
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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