S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 14 No.1 (1973)
Diphtheria 독소생산 기전에 관한 연구
Studies on the Mechanism of Toxin Production in Corynebacterium Diphtheriae
|dc.identifier.citation||Seoul J Med, Vol.14 No.1, pp. 1-5||-|
|dc.description.abstract||Corynebacterium dipheriae produces potent exotoxin
which acts a major role in the pathogenesis of diphtheria.
To understand the pathogenesis of diphtheria•
we must explain how the organisms become established
in the local lesion. understand the conditions
under which toxin is synthesized and released by the
C. diphtheriae and elucidate the nature of the toxin
molecule and the mechanism by which it kills the
Diphtheria toxin is only synthesized by the strains
of C. diphtheriae which are lysogenic for particular
bacteriophage and which are growing under conditions
of limiting iron content in the culture media. The
toxin is released into the external culture media as
it is formed during the growth and only trace can
be detected from the bacteria themselves.
Barksdale and his co-worker suggest that under
certain conditions induction of prophage to the
vegetative state by ultraviolet radiation may accelerate
the release of toxin and enhance its yield several
fold. Pappenheimer and his co-worker showed that
toxin protein was synthesized de nove from amino
acid by growi ng organisms.
In the present work we have followed intracellular
and extracellular toxin production. pH change. and
growth of C. diphtheriae PW 8 growing in iron free
and iron containing medium. The results are summerized
1. The highest toxin production was obtained at
10 hour culture in pH corrected iron free medium.
The toxin production rate at 10 hour was 3.4 Lf/
2. In the pH uncorrected iron free medium toxin
production was slow but accumulated toxin value was
the same as that in pH corrected iron free medium
after 35 hour culture.
3. 10' MRD/ml of toxin was detected in iron
containing culture fluid.
4. Intracellular toxin of iron free culture was
2xl0' MRD/mg protein and that of iron containing
culture was sxio' MRD/mg protein.
|dc.title||Diphtheria 독소생산 기전에 관한 연구||-|
|dc.title.alternative||Studies on the Mechanism of Toxin Production in Corynebacterium Diphtheriae||-|
|dc.citation.journaltitle||서울 의대 잡지||-|
|dc.citation.journaltitle||서울 의대 학술지||-|
|dc.citation.journaltitle||Seoul Journal of Medicine||-|
- Appears in Collections:
- College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 14 No.1 (1973)