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Vero cells expressing porcine circovirus type 2-capsid protein and their diagnostic application

Cited 5 time in Web of Science Cited 5 time in Scopus
Authors
Kim, Yeon-Hee; Kweon,Chang-Hee; Kang, Seung-Won; Oh, Yoon I; Song. Jae Young; Lee. Kyoung Ki; Park, Se Chang
Issue Date
2013-12
Publisher
Elsevier B.V.
Citation
Journal of Virological Methods Vol.194 No.1-2, pp. 26-32
Keywords
복합학Porcine circovirus type 2Lentiviral transductionQuantitative IFABoar
Abstract
Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in swine. Although the incidences of PCV2-related diseases are ubiquitous throughout the world, the serological tools are rather limited, mainly because the virus does not induce any cytopathic effects in cells.

The purpose of this study was to develop a rapid, sensitive and easy quantitative immunofluorescence assay (QIFA) using the recombinant PCV2 nucleocapsid protein (NCP) for the detection of PCV2-specific antibodies in pig sera. The recombinant PCV2 NCP was expressed in Vero cells by a lentivirus system. The performance of QIFA using these Vero cells as a diagnostic antigen was compared with currently available C-ELISA and I-ELISA; the relative sensitivity turned out to range from 92.5% up to 99.3%. The relative specificity was 93.3% when compared to C-ELISA as the gold standard. The serological experiment also indicated the inverse relationship between QIFA and the viral load in serum, semen, feces samples from 7 PCV2-positive boars. In addition, the PCV2 sequence detected from bone marrow cells shows 99% of sequence identity with PCV2 genome, confirming the infectivity of PCV2.
ISSN
0166-0934
Language
English
URI
https://hdl.handle.net/10371/83702
DOI
https://doi.org/10.1016/j.jviromet.2013.07.030
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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