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Role of interleukin-6 in the control of DNA synthesis of hepatocytes: involvement of PKC, p44/42 MAPKs, and PPARdelta
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Suh, Han Na | - |
dc.contributor.author | Lee, Sang Hun | - |
dc.contributor.author | Lee, Min Young | - |
dc.contributor.author | Lee, Yu Jin | - |
dc.contributor.author | Lee, Jang-Hern | - |
dc.contributor.author | Han, Ho Jae | - |
dc.date.accessioned | 2009-09-03T22:36:23Z | - |
dc.date.available | 2009-09-03T22:36:23Z | - |
dc.date.issued | 2008-12-09 | - |
dc.identifier.citation | Cell Physiol Biochem 2008;22(5):673-84 | en |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8603 | - |
dc.description.abstract | Interleukin-6 (IL-6) is a pleiotropic cytokine with a pivotal role in normal hepatic growth and liver regeneration. Therefore, in the present study, we examined the effect of IL-6 on cell proliferation and the related signaling pathways in primary cultured chicken hepatocytes. IL-6 increased the level of [3H]thymidine incorporation in a time ( 6 hr)- and a dose ( 0.1 ng/ml)-dependent manner. Indeed, IL-6 increased the number of BrdU-positive cells and the total number of cells. IL-6 (10 ng/ml) increased the level of IL-6R and glycoprotein (gp) 130 (IL-6R) protein expression, Janus Kinase (JAK) 2, signal transducer and activator of transcription (STAT) 3, PKC, p44/42 MAPKs phosphorylation, and PPAR protein expression. Inhibition of each pathways blocked IL-6-induced [3H]thymidine incorporation increase. IL-6 increased c-fos, c-jun, and c-myc proto-oncogene mRNA levels and the percentage of cells in the S phase according to fluorescence-activated cell sorter (FACS) analysis. IL-6-induced G1/S phase progression was inhibited by AG 490 (2x10-5 M, JAK2 inhibitor), a STAT3 inhibitor peptide (10-5 M), bisindolylmaleimide I (10-6 M, PKC inhibitor), PD 98059 (10-5 M, p44/42 MAPKs blocker), or PPAR-specific small interfering RNAs (siRNAs). In conclusion, IL-6 stimulates the proliferation of primary cultured chicken hepatocytes through PKC, p44/42 MAPKs, and PPAR pathways. | en |
dc.description.sponsorship | This study was supported by a Grant from
Korea Rural Development Administration (BioGreen 21 Program, 20050301-034-487-007-0300). The authors acknowledge a graduate fellowship provided by the Ministry of Education, Science and Technology through the Brain Korea 21 Project, Korea. | en |
dc.language.iso | en | en |
dc.publisher | Karger | en |
dc.subject | Interleukin-6 | en |
dc.subject | PKC | en |
dc.subject | p44/42 MAPKs | en |
dc.subject | PPARδ | en |
dc.subject | Hepatocytes proliferation | en |
dc.title | Role of interleukin-6 in the control of DNA synthesis of hepatocytes: involvement of PKC, p44/42 MAPKs, and PPARdelta | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 서한나 | - |
dc.contributor.AlternativeAuthor | 이상훈 | - |
dc.contributor.AlternativeAuthor | 이민영 | - |
dc.contributor.AlternativeAuthor | 이유진 | - |
dc.contributor.AlternativeAuthor | 이장헌 | - |
dc.contributor.AlternativeAuthor | 한호재 | - |
dc.identifier.doi | 10.1159/000185551 | - |
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