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Systemic cytokine profiles of mice vaccinated with naked DNAs encoding six open reading frame antigens of porcine circovirus type 2 (PCV2)

Cited 19 time in Web of Science Cited 20 time in Scopus
Issue Date
2008-05-23
Publisher
Elsevier
Citation
Res. Vet. Sci. 85, 503-509
Keywords
Cytokine profilePCV2ORFs
Abstract
The objective of this study was to characterize the murine immune response to vaccination with DNAs encoding each of six porcine circovirus type 2 (PCV2) open reading frames (ORFs). After intramuscular vaccination with the naked DNAs, blood was taken on days post-infection (DPI) 7 and 35 and subjected to Bio-Plex cytokine assays. ORF3 elicited high levels of the pro-inflammatory cytokine TNF-α (P < 0.001) and decreased IL-12 levels (P < 0.001) on DPI 7, and was lethal for nine of the 15 vaccinated mice, which died on DPIs 4, 6, 9, and 10. The remaining six mice showed general prostration but then recovered. The clinical signs correlated with the systemic TNF-α and IL-12 levels. ORF1 vaccination elevated T-helper (Th)1 (IFN-γ; P < 0.001) and Th2 (IL-13; P < 0.05) cytokine levels on DPI 35, while ORF2 markedly elevated the expression of the humoral immunity- and Th-2-related cytokine IL-10 (P < 0.001) on DPI 35. These observations provide insights into the immune responses generated by each PCV2 ORF.
ISSN
0034-5288
Language
English
URI
https://hdl.handle.net/10371/8604
DOI
https://doi.org/10.1016/j.rvsc.2008.03.006
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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