Browse

Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts

Cited 8 time in Web of Science Cited 10 time in Scopus
Authors
Choresca Jr., Casiano H.; Koo, Ok Jae; Oh, Hyun Ju; Hong, So Gun; Gomez, Dennis K.; Kim, Ji Hyung; Lee, Byeong Chun; Park, Se Chang
Issue Date
2008-10-17
Publisher
Elsevier
Citation
Cell Biol Int 2009;33:65-70
Keywords
Cell cycle synchronizationG0/G1 phaseGoldfishSerum starvationCulture to confluenceRoscovitine
Abstract
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 μM) induce cell cycle arrest at the G0/G1 phase.
ISSN
1065-6995
Language
English
URI
http://hdl.handle.net/10371/8605
DOI
https://doi.org/10.1016/j.cellbi.2008.09.015
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse