S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Civil & Environmental Engineering (건설환경공학부) Journal Papers (저널논문_건설환경공학부)
PAH utilization by Pseudomonas rhodesiae KK1 isolated from a former manufactured gas plant site
- Kahng, Hyungyeel; Nam, Kyoungphile; Kukor, Jerome J.; Yoon, Byoungjun; Lee, D.-H.; Oh, Duckchul; Kam, Sangk; Oh, Kyeheon
- Issue Date
- Springer Verlag
- Appl Microbiol Biotechnol 60:475-480
- Pseudomonas rhodesiae KK1 was isolated from a former manufactured-gas plant site, due to its ability to grow rapidly in a mixture of polycyclic aromatic hydrocarbons (PAHs). Radiorespirometric analysis revealed that strain KK1 was found to be able to mineralize anthracene, naphthalene and phenanthrene. Notably, phenanthrene-grown cells were able to mineralize anthracene much more rapidly than naphthalene-grown cells. Comparative analysis of amino acid sequences from 17 randomly selected dioxygenases capable of hydroxylating unactivated aromatic nuclei indicated that the enzymes for catabolism of PAHs, such as naphthalene and phenanthrene, might exist redundantly in strain KK1. Northern hybridization for cells grown on naphthalene or phenanthrene, using the putative naphthalene or phenanthrene dioxygenase gene fragment as a probe, suggested that the enzyme for naphthalene catabolism might share some homology in deduced amino acid sequences with phenanthrene dioxygenases. Also, it was found that three lipids (17:0 cyclo, 18:1 ω7c, 19:0 cyclo) increased in response to both naphthalene and phenanthrene, while the shift of other lipids varied from substrate to substrate.
- 0175-7598 (print)
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