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Fabrication and Evaluation of Osteoblastic Differentiation of Human Mesenchymal Stem Cells on Novel CaO-SiO2-P2O5-B2O3 Glass-Ceramics
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jae Hyup | - |
dc.contributor.author | Seo, Jun-Hyuk | - |
dc.contributor.author | Lee, Kyung Mee | - |
dc.contributor.author | Ryu, Hyun-Seung | - |
dc.contributor.author | Baek, Hae-Ri | - |
dc.creator | 이재협 | - |
dc.date.accessioned | 2014-02-24T00:26:40Z | - |
dc.date.available | 2014-02-24T00:26:40Z | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | Artificial Organs Vol.37 No.7, pp. 637-647 | - |
dc.identifier.issn | 0160-564X | - |
dc.identifier.uri | https://hdl.handle.net/10371/90960 | - |
dc.description.abstract | Apatite-wollastonite glass-ceramics have high mechanical strength, and CaO-SiO2-B2O3 glass-ceramics showed excellent bioactivity and high biodegradability. A new type of CaO-SiO2-P2O5-B2O3 system of bioactive glass-ceramics (BGS-7) was fabricated, and the effect and usefulness was evaluated via bioactivity using simulated body fluid and human mesenchymal stem cells (hMSCs). The purpose of this study was to compare BGS-7 and hydroxyapatite (HA) using hMSCs in order to evaluate the bioactivity of BGS-7 and its possibility as a bone graft extender. Alkaline phosphatase (ALP) staining, ALP activity, cell proliferation 3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, Alizarin Red-S (AR-S) staining, calcium levels, the mRNA expression of ALP, osteocalcin, osteopontin, and runt-related transcription factor 2 (runx-2) using reverse-transcription polymerase chain reaction (RT-PCR) and the protein expression of osteocalcin and runx-2 using Western blot were measured by transplanting hMSC onto a tissue culture plate, HA, and BGS-7. The ALP staining and AR-S staining of BGS-7 was greater than that of HA and control. The ALP value of BGS-7 was significantly higher than that of HA and control. The MTS results showed that BGS-7 had a higher value than the groups transplanted onto HA and control on day 15. The calcium level was higher than the control in both HA and BGS-7, and was especially high in BGS-7. There were more mineral products on BGS-7 than on the HA when analyzed by scanning electron microscopy. The mRNA expression of ALP, osteopontin, osteocalcin, and runx-2 were higher on BGS-7 than on HA and the control when analyzed by RT-PCR. The relative gene expression of osteopontin and runx-2 were found to be higher on BGS-7 than on HA and the control by Western blot. Accordingly, it is predicted that BGS-7 would have high biocompatibility and good osteoconductivity, and presents a possibility as a new bone graft extender. | en |
dc.description.sponsorship | This work was supported by a Grant-in-Aid from the Seoul Development Institute (Seoul R&BD Program, No. ST100012). | - |
dc.language.iso | en | en |
dc.publisher | International Center for Artificial Organs and Transplantation and Wiley Periodicals | en |
dc.subject | 복합학 | en |
dc.subject | CaO-SiO2-P2O5-B2O3 glass-ceramics | - |
dc.subject | Osteoblastic differentiation | - |
dc.subject | Osteoconductivity | - |
dc.subject | Bone graft extender | - |
dc.title | Fabrication and Evaluation of Osteoblastic Differentiation of Human Mesenchymal Stem Cells on Novel CaO-SiO2-P2O5-B2O3 Glass-Ceramics | en |
dc.type | Article | - |
dc.author.alternative | 이재협 | - |
dc.author.alternative | 서준혁 | - |
dc.author.alternative | 이경미 | - |
dc.author.alternative | 류현승 | - |
dc.author.alternative | 백나리 | - |
dc.identifier.doi | 10.1111/aor.12027 | - |
dc.citation.journaltitle | Artificial Organs | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000007273/3 | - |
dc.description.srnd | SEQ:3 | - |
dc.description.srnd | PERF_CD:SNU2013-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000007273 | - |
dc.description.srnd | ADJUST_YN:Y | - |
dc.description.srnd | EMP_ID:A077517 | - |
dc.description.srnd | DEPT_CD:801 | - |
dc.description.srnd | CITE_RATE:1.964 | - |
dc.description.srnd | FILENAME:첨부된 내역이 없습니다. | - |
dc.description.srnd | DEPT_NM:의학과 | - |
dc.description.srnd | EMAIL:spinelee@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2013-01/102/0000007273/3 | - |
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