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The Rhizomes of Acorus gramineus and the Constituents Inhibit Allergic Response In vitro and In vivo

Cited 7 time in Web of Science Cited 8 time in Scopus
Authors
Lim, Hyun; Lee, Seung Young; Lee, Kang Ro; Kim, Yeong Shik; Kim, Hyun Pyo
Issue Date
2012-09
Publisher
Korean Society of Applied Pharmacology
Citation
Biomolecules & Therapeutics Vol.20 No.5, pp. 477-481
Keywords
의약학Acorus gramineusβ-Asarone2,3-Dihydro-4,5,7-trimethoxy-1-ethyl-2-methyl-3-(2,4,5-trimethoxyphenyl)indene5-Lipoxygenaseβ-HexosaminidaseAnti-allergy
Abstract
The rhizomes of Acorus gramineus have frequently been used in traditional medicine mainly for sedation as well as enhancing brain function. In this study, the anti-allergic activity of A. gramineus was investigated. The 70% ethanol extract of the rhizomes of A. gramineus was found to inhibit the allergic response against 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat basophilic leukemia (RBL)-1 cells and β-hexosaminidase release from RBL-2H3 cells with IC50's of 48.9 and >200 μg/ml, respectively. Among the 9 major constituents isolated, β-asarone, (2R,3R,4S,5S)-2,4-dimethyl-1,3-bis (2',4',5'-trimethoxyphenyl)tetrahydrofuran (AF) and 2,3-dihydro-4,5,7-trimethoxy-1-ethyl-2-methyl-3-(2,4,5-trimethoxyphenyl)indene (AI) strongly inhibited 5-LOX-catalyzed LT production in A23187-treated RBL-1 cells, AI being the most potent (IC50=6.7 μM). Against β-hexosaminidase release by antigen-stimulated RBL-2H3 cells, only AI exhibited strong inhibition (IC50=7.3 μM) while β-asarone and AF showed 26.0% and 39.9% inhibition at 50 μM, respectively. In addition, the ethanol extract of A. gramineus showed significant inhibitory action against the hapten-induced delayed hypersensitivity reaction in mice by oral administration at 200 mg/kg. Therefore, it is suggested that A. gramineus possesses anti-allergic activity and the constituents including β-asarone and AI certainly contribute to the anti-allergic activity of the rhizomes of A. gramineus.
ISSN
1976-9148 (print)
2005-4483 (online)
Language
English
URI
https://hdl.handle.net/10371/91114
DOI
https://doi.org/10.4062/biomolther.2012.20.5.477
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College of Pharmacy (약학대학)Dept. of Manufacturing Pharmacy (제약학과)Journal Papers (저널논문_제약학과)
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