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Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-α and IL-1.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ho-Joong | - |
dc.contributor.author | Yeom, Jin S. | - |
dc.contributor.author | Koh, Yong-Gon | - |
dc.contributor.author | Yeo, Jee-Eun | - |
dc.contributor.author | Kang, Kyoung-Tak | - |
dc.contributor.author | Kang, Young-Mi | - |
dc.contributor.author | Chang, Bong-Soon | - |
dc.contributor.author | Lee, Choon-Ki | - |
dc.creator | 염진섭 | - |
dc.date.accessioned | 2014-04-18T02:22:02Z | - |
dc.date.available | 2014-04-18T02:22:02Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | Journal of Orthopaedic Research Vol.32 No.4, pp. 551-556 | - |
dc.identifier.issn | 0736-0266 (print) | - |
dc.identifier.issn | 1554-527X (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/91405 | - |
dc.description.abstract | The purpose of this study was to investigate the anti-inflammatory effect of platelet-rich plasma (PRP) with collagen matrix on human nucleus pulposus (NP) cell in response to pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). NP cells from human disks were cultured in a monolayer and maintained in the collagen matrix prior to the addition of recombinant human IL-1 and TNF-α. After applying IL-1 and TNF-α, PRP prepared by using a commercially available platelet concentration system was added. The response was investigated using real-time PCR for mRNA expression of type II collagen, aggrecan, matrix metalloproteinase-3 (MMP-3), and cyclooxygenase-2 (COX-2). The combination of IL-1β and TNF-α led to decrease of matrix synthesis gene expression such as collagen type II and aggrecan and increase of the degradation gene expression of COX-2 and MMP-3, compared to the control. Consecutive PRP exposure significantly recovered the down-regulated gene expression of collagen type II and aggrecan and significantly reduced the increased MMP-3 and COX-2 gene expression, compared to that of control groups with pro-inflammatory cytokines. The administration of PRP with collagen matrix markedly suppressed cytokine-induced pro-inflammatory degrading enzymes and mediators in the NP cell. It also rescued gene expression concerning matrix synthesis, thereby stabilizing NP cell differentiation. | en |
dc.language.iso | en | en |
dc.publisher | Wiley Periodicals | en |
dc.subject | 의약학 | en |
dc.subject | platelet-rich plasma | - |
dc.subject | nucleus pulposus cell | - |
dc.subject | interleukin-1 | - |
dc.subject | tumor necrosis factor-α | - |
dc.title | Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-α and IL-1. | en |
dc.type | Article | - |
dc.author.alternative | 김호중 | - |
dc.author.alternative | 염진섭 | - |
dc.author.alternative | 고영곤 | - |
dc.author.alternative | 여지은 | - |
dc.author.alternative | 강경택 | - |
dc.author.alternative | 강영미 | - |
dc.author.alternative | 장봉순 | - |
dc.author.alternative | 이춘기 | - |
dc.identifier.doi | 10.1002/jor.22532 | - |
dc.citation.journaltitle | Journal of Orthopaedic Research | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2014-01/102/0000004226/1 | - |
dc.description.srnd | SEQ:1 | - |
dc.description.srnd | PERF_CD:SNU2014-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000004226 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A079510 | - |
dc.description.srnd | DEPT_CD:801 | - |
dc.description.srnd | CITE_RATE:2.875 | - |
dc.description.srnd | FILENAME:j orthop res-2014_kim_anti inflammatory effect of platelet rich plasma on nucleus pulposus cells.pdf | - |
dc.description.srnd | DEPT_NM:의학과 | - |
dc.description.srnd | EMAIL:spine@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2014-01/102/0000004226/1 | - |
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