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Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-α and IL-1.

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dc.contributor.authorKim, Ho-Joong-
dc.contributor.authorYeom, Jin S.-
dc.contributor.authorKoh, Yong-Gon-
dc.contributor.authorYeo, Jee-Eun-
dc.contributor.authorKang, Kyoung-Tak-
dc.contributor.authorKang, Young-Mi-
dc.contributor.authorChang, Bong-Soon-
dc.contributor.authorLee, Choon-Ki-
dc.creator염진섭-
dc.date.accessioned2014-04-18T02:22:02Z-
dc.date.available2014-04-18T02:22:02Z-
dc.date.issued2014-04-
dc.identifier.citationJournal of Orthopaedic Research Vol.32 No.4, pp. 551-556-
dc.identifier.issn0736-0266 (print)-
dc.identifier.issn1554-527X (online)-
dc.identifier.urihttps://hdl.handle.net/10371/91405-
dc.description.abstractThe purpose of this study was to investigate the anti-inflammatory effect of platelet-rich plasma (PRP) with collagen matrix on human nucleus pulposus (NP) cell in response to pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). NP cells from human disks were cultured in a monolayer and maintained in the collagen matrix prior to the addition of recombinant human IL-1 and TNF-α. After applying IL-1 and TNF-α, PRP prepared by using a commercially available platelet concentration system was added. The response was investigated using real-time PCR for mRNA expression of type II collagen, aggrecan, matrix metalloproteinase-3 (MMP-3), and cyclooxygenase-2 (COX-2). The combination of IL-1β and TNF-α led to decrease of matrix synthesis gene expression such as collagen type II and aggrecan and increase of the degradation gene expression of COX-2 and MMP-3, compared to the control. Consecutive PRP exposure significantly recovered the down-regulated gene expression of collagen type II and aggrecan and significantly reduced the increased MMP-3 and COX-2 gene expression, compared to that of control groups with pro-inflammatory cytokines. The administration of PRP with collagen matrix markedly suppressed cytokine-induced pro-inflammatory degrading enzymes and mediators in the NP cell. It also rescued gene expression concerning matrix synthesis, thereby stabilizing NP cell differentiation.en
dc.language.isoenen
dc.publisherWiley Periodicalsen
dc.subject의약학en
dc.subjectplatelet-rich plasma-
dc.subjectnucleus pulposus cell-
dc.subjectinterleukin-1-
dc.subjecttumor necrosis factor-α-
dc.titleAnti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-α and IL-1.en
dc.typeArticle-
dc.author.alternative김호중-
dc.author.alternative염진섭-
dc.author.alternative고영곤-
dc.author.alternative여지은-
dc.author.alternative강경택-
dc.author.alternative강영미-
dc.author.alternative장봉순-
dc.author.alternative이춘기-
dc.identifier.doi10.1002/jor.22532-
dc.citation.journaltitleJournal of Orthopaedic Research-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2014-01/102/0000004226/1-
dc.description.srndSEQ:1-
dc.description.srndPERF_CD:SNU2014-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000004226-
dc.description.srndADJUST_YN:N-
dc.description.srndEMP_ID:A079510-
dc.description.srndDEPT_CD:801-
dc.description.srndCITE_RATE:2.875-
dc.description.srndFILENAME:j orthop res-2014_kim_anti inflammatory effect of platelet rich plasma on nucleus pulposus cells.pdf-
dc.description.srndDEPT_NM:의학과-
dc.description.srndEMAIL:spine@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2014-01/102/0000004226/1-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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