동종 및이종 부신조직의 반복주사에 의한 실험적 부신염에 관한 연구

Abstract

Immunological adrenal injury was produced in rabbits by repeated injections of pooled homologous and heterologous adrenal tissue homogenates respectivly, incorporated into Freunds complete adjuvant. to elucidate the histopathologic features and determine the mechanisms of adrenal cell damage. 1. Various degree of widespread adrenal lesions were found in 14 of 18 animals receiving homologous tissue antigen and to a lesser extent in one out of 9 animals injected with heterologous pooled antigen 2. Those adrenal lesions were characterized by progressive alteration of both inflammatory and retrogressive changes in the deeper cortex and medulla; the earlier changes consisted of perisinu • soidal cellular hyperplasia in the deeper layer of cortex, and appeared on the 6th week following the first injection, with subsequent confluence 'of such lesions by prolongation of experimental duration. 3. Major histologic changes included earlier focal perisinusoidal lithoral cell proliferation followed by mononuclear cell aggregations, but soon with intense inflammatory process, cytoplasmic eosinophilia, nuclear pyknosis and cytolysis in the later group 4, The adrenal cortex illustrated selective localiza tion of such lesions in the deeper portion of zona fasciculata and reticularis, being most extensive in the cortico-medullary junction. No lesions were found in either zona glomerulosa or outer fasciculata; such a selective involvement was probably attributable to the development of sinusoidal structures and lithoral cells. 5. Medullary changes, though mainly affecting cortico-medullary junction, were always associated with deeper cortical lesions. and none of those animals disclosed isolated medullary involvement. It was anticipated that ectopic cells as well as simultaneous administration of medullary tissue antigen might playa significant role