S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 04 No.1 (1963)
정상 및 Alloxan당뇨병 흰쥐의 뇌절편에 있어서 C14-초성포도당 및 포도당의 대사에 관한 실험적 연구
Metabolism of C14-labeled Pyruvate and Glucose by Brain-Slices of Normal and Alloxan-Diabetic Rats.
- 장신요; 장가용; 이상돈
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.4 No.1, pp. 1-8
- Brain slices of 58 normal and alloxan-diabetic rats
were divided into 4 groups. Each group was incubated
for a period of 5 hours with incubation mixture
consisting of 15 C.c. of phosphate buffer to which
were added C1L glucose or C14-pyruvatc and 15 C.c
of oxygen. During 5 hours of incubation period. the
incubation media and gas phase were replaced with
fresh media and oxygen at hourly intervals. This
procedure made it possible to obtain hourly samples to determine the concentration of medium substrates
and the amount and radioactivity of CO2 produced by
the brain slices during the experimental period. It
also had the advantage of presenting the tissue with
a substrate of essentially constant concentration and
Data obtained from the C14-pyruvate incubation
experiment, in the first and second group , and from
the ClL glucose incubation experiment in the third and
fourth group , were summarized as the follows.
1. In the ClL pyruvate incubation experiment, pyruvate
disappearance rates were mean of 10.7 μM/hr/g
in the first control group , and 12. 2 μM/hr/g in
the second alloxan-diabetic group. The lactate accumulation
rates in both groups were mean of 5. 8 and
5.1 μM/hr/g respectively.
Total CO2 production rates in both groups were
mean of 49. 7 and 63. 4 μM/hr/g respectively, showing
a slightly higher value in the second group.
However, since RSA, which represents the fraction
of C14-02 derived from medium CILpyruvate to total
CO2 production rate, was slightly hiher in the control
group than the diabetic-group, there were little diffe•
rences in C02 production rates derived from medium
CILpyruvate of both groups.
2. In the C14-glucose incubation experiment, ClL
glucose disappearance rates decreased about one half
of the control in the diabetic group , showing a mean
of 44.1 μM/hr/g in the third control group and
20.0 μM/hr/g in the fourth alloxan-diabetic group.
Since there were not significant differences in total
CO2 production rates and values in RSA, Cl4-0 2 production
rates derived from medium clιglucose have
been found to be almost the same value in both
From the data described above, insulin cannot have
an influence on all the steps of carbohydrate metab
olism in the brain tissue. However there is evidence
that insulin exhibits the effect on membrane transport
of glucose as found in the other tissues.