회충의 C14-포도당대사에 관한 연구

Abstract

Adult forms of Ascaris lumbricoides suum employed in this experiment were obtained from a local abattoir. The worms were selected and washed several times in normal sterilized saline solution. These selected fifteen worms were then plilced in special incubation flasks containing each 50 cc. of KrebsRinger phosphate buffer (pH, 7.4) . This medium was added by uniformely labeled Cl4- glucose so as to contain glucose concentration of 200mg. per cent and 2~2.5 μC of radioactivities. The worms were allowed to incubate for 6 hours in the incubator at 38°C. After incubation period , respiratory CO2 samples from central well of incubation flask were analyzed for total CO2 production rate and their specific activity of respiratory CO2, Glycogen samples isolated from worms were analized for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. The glucose uptake rate was determined by analyzing the difference of the glucose concentration in a medium before and after incubation period. Radioacttivities of these series of experiments were counted by an endwindow GeigerMuller counter asan infmitively thin samples. The quantitative analysis of C14-glucose utilized by Ascaris lumbricoides were summerized as the followmg. 1. The glucose uptake rate by Ascaris lumbricoides was a mean value of 6.32土1.07 μ lVI/hr/g. ， and total CO2 production rates by the worms averaged 6.58士 0.61 μM/hr Igm. The relative specific activity of respiratory CO2 (R.S.A.co2) varied from 10.3 to 73.7 per cent showing a mean value of 33.9 土4.7 per cent. Thus, a mean of 33.9 per cent of total CO2 production rates 、"，a s originated from C14-glucose in a medium, therefore the rate of CO2 production derived from medium Cl4.glucose was a mean of 2 .42土0.41 μM/hr/g. in the 15 cases of Ascaris. 2. The tissue concentration of glycogen in Ascaris was a mean of 59.5士50 mg/g. , and the turnover rate of glycogen pool yielded values ranging from 0.10 to 0.70%/hr. with a mean of 0 .43土0.08%/hr. in 15 cases of experiments. The half time (t상 of glycogen turnover, which is the time interval required to replace the half of glycogen pool with medium (14-glucose. gave value of a mean of 1 1.95 士2.37 days. In comaprison with the turnover rate of glycogen in carcass and sexual organs of female worms, the turnover rate of sexual organs seems like to be slightly higher value than that of carcass. 3. The a\'(~rage value of 6.82±0.96 per cent(R.G.D. C02) of glucose utilized by the worms from the medium C14-glucose was oxidized to respiratory CO2 and a mean value of 24 .4 土3.2 per cent (R.G.D.gly) of glucose was incorporated to the glycogen. These data account for that at least 31.2 per cent of the utilized glucose by the worms participated in furnishing the oxidation into respiratory CO2 and the synthetic process into glycogen. According to the above data of the experiement, it is suggested in the metabolic process of glucose by the Ascaris that the synthetic process into the glycogen is more active than the oxidative process into the respiratory CO2.