S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 04 No.4 (1963)
Glycyrrhizin이 각종약물의 마우스 치사율에 미치는 영향
Effects of Gly cyr r h izin on Mortality Rate of Mouse Challenged with Poisons
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.4 No.4, pp. 69-87
- It has been suggested that glycyrrhizin, the active
principle of Glycyrrhiza, has the antidotal action
against various toxic substances. The effects of glycyrrhizin
on mortality rate of mouse challenged with
various poisons were evaluated. The chemical studies
were also carried out to determine the action mechanism
of glycyrrhizin. Following were the results:
1. Oral administration of glycyrrhizin resulted in a
marked decrease of mortality rate of mouse challen
ged with strychnine nitrate, atropine sulfate and
physostigmine salicylate, while no effects against aconitine.
morphine hydrochloride, arsenic pentoxide
and ephedrine hydrochloride.
2. Intraperitoneal administration of glycyrrhizin
resulted in a decrease of mortality rate of mouse
challenged with strychnine nitrate. physostigmine salicylate,
picrotoxin, tetradotoxin, diphtheria toxin and
tetanus toxin. especially in strychnine and physostigmine
poisonings. However. no effect was observed
against cocaine hydrochloride. atropine sulfate, aconitine.
morphine hydrochloride. digitoxin, procaine
hydrochloride. barbital sodium. histamine diphosphate,
mercury bichloride and arsenic pentoxide.
The mortality rate of mouse challenged with ca·
Heine and sodium benzoate. ephedrine hydrochloride
and tolazoline hydrochloride was, on the contrary,
3. Fifty percent lethal doses of mouse were: 1.555
mg/kg with strychnine nitrate alone , 2.295mg/kg with
strychnine nitrate and glycyrrhizin. 0.950 mg/kg with
physostigmine salicylate alone. 1.285 mg/kg with phy
sostigmine salicylate and glycyrrhizin,6.39 mg/kg with
picrotoxin alone. 8.14 mg/kg with picrotoxin and
glycyrrhizin. 662.5 mg/kg with caffeine and sodium
benzoate alone , 445.5 mg/kg with caffeine and sodium
benzoate and glycyrrhizin , and 515.0 mg/kg with ephedrine hydrochloride alone , 498.0 mg/kg with ephedrine
hydrochloride and glycyrrhizin,
4. Similar effects were also observed in mortality
rate of gold-fish.
5. The effects of glycyrrhizin were compared with
those of glycyrrhetinic acid and glucuronic acid ,
hydrolytes of glycyrrhizin. The principal action of
glycyrrhizin appeared to originate from glycyrrhizin
6. Slower absorption of Fe59 in mouse occurred
when the mixture of isotope and glycyrrhizin was
given orally, while no difference in the rate of absorption
was observed upon subcutaneous administration.
7. Glycyrrhizin was very active in blocking Meyer s
reaction of various alkaloids
8. Glycyrrhizin appeared to decrease the detectable
amounts of alkaloids precipitated by ammonium reineckate;
to a greater extent in strychnine nitrate and
atropine sulfate, and to a lesser extent in aconitine
and cocaine hydrochloride.
9. When glycyrrhizin was added to the saturated
alkaloid solutions, the changes in solubilities occurred;
considerable decrease in strychnine and atropine, no
changes in cocaine and aconitine, and slight increase
in ephedrine and caffeine.
10. When glycyrrhizin was directly mixed with
various alkaloids , precipitating reaction occurred only
11. From these chemical studies, glycyrrhizin ap
peared to form complexes with various agents and to
exert influences on their solubilities.