S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 13 No.1 (1972)
암조직에 있어서 C14-함수탄소의 단백질 합성과정에 관한 실험
Incorporation of C14-labeled carbohydrate into protein in the Walker 256 tumor
- 김윤선; 이상돈
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.13 No.1, pp. 35-40
- homogenates of tumor, liver, brain and kidney
tissues excised from Walker 256 tumor animals were
divided to 3 aliquots and incubated with C"-l, CU-2
and C"-3-lactate media respectively.
C“02 yields from each aliquot of experiment were
compared to determine the oxidative pathway of
intermedially metabolite of sugar and also incorpora•
tion of C"•lactate into tissue protein.
Results obtained were as follow.
1) Total C02 production rate of tumor tissue was
averaged 10. 2±3. 2uM/hr/gm and those of control
tissues were showed 27. 8±7. 2 in the liver, 44.0±
6.8 in the brain and 42.7±55 uM/hr/gm in the
kidney tissue respectively.
These data impressed general oxidative metabolism
in the tumor tissues were inhibited compared with
those of control tissues.
2) C02 yields from each carbon of lactate in the
tumor and control tissues were compared.
C02 yields from C-1 carbon of lactate were always
larger than those from C-2 and C-3 carbon of
lactates. in every tissues. However this difference
in the tumor tissue is remarkable as compared with
normal tissues. these data suggested that carboxyl
carbon of lactate was easily oxidized into C02 in the
tumor tissue as In the normal tissues but further
oxidation of C•2 and C-3 carbon of lactate, which
Is acetate unit, were remarkably inhibited in the
3) Incorporation of radioactivities of each carbon
of lactate into tissue protein was observed in order to trace the fate of 2 carbon compound which is
inhibited further oxidation into C02 in the tumor
tissue. Incorporations of radioactivities into protein
in the CJI-I-Iactate incubation experiments were
always lower than those in C"-2 and C"-3-lactate
incubation experiments in tumor and control tissues
but remarkable difference was observed in the tumor
tissue as comparing with control tissue. Thus means
remaining 2 carbon compound after decarboxylation
of lactate were participate to protein synthesis relatively
larger part in the tumor tissues than in the
From the above data we suggested metabolic
pathway of lactate in the tumor tissue as in the