Detailed Information

항체 생성세포의 발생기원에 관한 연구(1) : Studies on antibody forming cells I. Histochemical localization of injected horseradish peroxidase in rat spleen.

Cited 0 time in Web of Science Cited 0 time in Scopus

백상호; 심재도; 장가용; 이광호

Issue Date
서울대학교 의과대학
Seoul J Med, Vol.13 No.1, pp. 61-67
Adult rats were injected intravenously with 20mg
of horseradish peroxidase. A series of rats were
sacrificed at intervals ranging from 1 minute to 24
hours, and spleens were processed for sensitive
histochemical reaction to localize exogenous peroxi
dase. Methyl green pyronin staining and acid phos•
phatase reaction were also applied to them singly or
combined with HRP staining.
Numerous HRP trapping ceIls were found throughout
the red pulp , junction of red and white pulp of the spleen , while in the white pulp, no evidence of
HRP positive ce11s were obtained.
Lysosome rich ce11s in both of white and red pulp
appeared positively by the acid phosphatase reaction,
but not a11 of them were positive in HRP staining.
In HRP-MGP double stained sections, HRP was
localized mainly in the macrophages of the splenic
cord and lining ce11s of the blood sinuses.
Uptake of exogenous antigen by the splenic macrophages
was recognized 1 minute after injection and
was increased progressively up to 2 hours. No trace
of HRP was observed in the ce11s or in the interce11ular
spaces of the white pulp.
Files in This Item:
Appears in Collections:


Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.