항체생성세포의 발생기원에 관한 연구 Ⅱ. 자기방사법을 이용한 항체형성세포의 기원에 관하여

Abstract

A development study of the plasma cells was performed on the spleen of the young adult rabbits, weighing from 1. 7 to 2. 0 Kg. , after a single injection of 1 ml. diphthelia toxoid (22 Lf/ ml. , from 5x recrystalized diphthelial toxin, ) intravenously. Control and injected animals both received intravenously O. 3μC of 3H-thymidine (specific activity 6.7 curies/mM) per gm. body weight on the 3rd day after the injection. Animals were sacrificed at various intervals(from 1st day to 14th day) and spleen of both groups (injected and control) stained with methyl-green pyronin were compared each other. One of the main changes found was in the periarterial lymphocyte sheath and perifollicular zone in the white pulp. An increase of large pyroninophilic cells labelled with 3H-thymidine was found in this area on the 3rd day after the injection of the antigen. On the 6th and 8th days, a decrease occurred in the proportion of these cells in this area, while some of the labelled plasma cell series were found in the junction of the red and white pulp. The other histological changes found were in the germinal center of the white pulp. An increase of the medium-sized lymphocytes and the large lymphocytes appeared in this area on the 6th day after the injection of the antigen and these cells were found incorporated with 3H-thymidine. And the changes of the germinal center persisted on the period during the subsequent 6th to 14th day. It suggests that the germinal center do not concern with the process of the antibody production and may playa role in the production of memory cells.