에르릿히 복수종양에 있어서 젖산 각 탄소산화과정의 분해 경로에 관한 연구 : Catabolic Pathway of Oxidative Metabolism of Carbon Atoms of Lactate in Ehrlich Ascites Tumor

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문일순; 이상돈

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서울대학교 의과대학
Seoul J Med, Vol.7 No.2, pp. 43-50
Metabolic pathway of carbon atoms of lactate in the
Ehrlich ascites tumor tissue homogenate was observed
in mice by means of labeled lactate. Tumor tissue homogenates
were incubated in medium containing either
one of CIL1, C1L2, or C14-3-lactate. Lactate concentration
in incubation medium was maintained at 50
mg%. At the end of incubation period, gas sample
and incubation medium were analyzed for total CO2
production rate, radio-activities of respiratory CO2 produced
, lactate uptake rate and pyruvate appearance
rate. The following results were obtained.
1. Mean value of lactate uptake rate was 2.07 μM/
hr/gm and pyruvate appearance rate was 0.039 μM/
hr/gm. The mean value of total CO2 production rate
was 15.1 μM/hr/gm. These values showed no difference
regardless of the shift of labeling position of lactate
carbon atom.
2. Respiratory C140 2 yield from OLI-lactate incubation
medium was 14.4% of the total CO2production
rate and this proportion was larger than that of from
0'-2 and OL3-lactate media. 0'02 yield from OL2
and 0'-3-lactate media were 0.74 and 0.65 % of the
total CO2 production rate, respectively. This shows
that carboxyl carbon of lactate contributes a larger
proportion in producing respiratory CO2 than a and ~
carbons of lactate.
3. The fraction of disappeared lactate from incubation
medium into respiratory CO: was expressed as re
lative lactate disappearance rate into CO2 (RLDc02)
and was averaged 38.3%. Relative lactate disappearance
rate into pyruvate (RLDpy) was 1. 9%. Therefore
, about 40 % of disappeared lactate were accounted
for by the conversion into respiratory CO2and pyruvate.
It is concluded that in the catabolic pathway of lactate
in ascites tumor tissue CO2 was easily produced
from carboxyl carbon of lactate by oxidative decarbo'
xylation as in the normal tissue , and further oxidation
of 2 carbon unit via the TCA cycle was remarkably
inhibited. On the other hand, from the fact there are
little differences in 0'02 yield between 0'-2 and OL
3-lactate medium group, it is reasonable to assume
that carbohydrate synthesis was also inhibited in the
ascites tumor tissue. Possible explanations for these
effects were briefly discussed.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 07 No.2 (1966)
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