S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 16 No.1 (1975)
적혈구 세포막의 Na+-K+ ATPase 및 Ca+ ATPase 활성에 관한 연구
The Effects of Various Experimental Conditions on Na+-K+ ATPase and Ca+ ATPase Activities of Red Blood Cell Membrane Fragments
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.16 No.1, pp. 76-80
- It has been shown that the red cell membrane
has an active mechanism for transporting
Ca++ out of red blood cells (Lee et al.
1969; Olson et al. 1969; Schatzman, 1966;
Schatzman et al. 1969). A Ca++-activated
ATPase, in the presence of Mg++(Ca++ ATPase)
, appears to provide the energy for the
Ca++ extrusion process (Schatzman et aI, 19
69; Vincenzi et al, 1967). On the other hand
it is well established that a NaLKLactivated
ATPase(NaLK+ ATPase) is an integral part
of the red cell membrane and provides the
energy for outward extrusion of Na+(Post et
al. 1960; Skou, 1965). In view of the apparent
localization of these two ATPases for
transport of Na+ and Ca++ in the cell membrane,
it would be of interest to study the
relationship between these ATPases.
In the present investigation, the effects of
various experimental conditions on Na+-K+
ATPase, on the one hand, and the Ca+十
ATPase, on the other, were studied, employing
red blood cell membrane fragments.
The results indicate that these two ATPases
share many common characteristics. but they are two distinctly different enzyme systems,
located in the same cellular membrane.