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Modification of PCNA by ISG15 Plays a Crucial Role in Termination of Error-Prone Translesion DNA Synthesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jung Mi | - |
dc.contributor.author | Yang, Seung Wook | - |
dc.contributor.author | Yu, Kyung Ryun | - |
dc.contributor.author | Ka, Seung Hyun | - |
dc.contributor.author | Lee, Seong Won | - |
dc.contributor.author | Seol, Jae Hong | - |
dc.contributor.author | Jeon, Young Joo | - |
dc.contributor.author | Chung, Chin Ha | - |
dc.creator | 정진하 | - |
dc.date.accessioned | 2014-10-29T07:19:34Z | - |
dc.date.available | 2014-10-29T07:19:34Z | - |
dc.date.issued | 2014-05 | - |
dc.identifier.citation | Molecular Cell, Vol.54 No.4, pp. 626-638 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://hdl.handle.net/10371/93475 | - |
dc.description.abstract | In response to DNA damage, PCNA is mono-ubiquitinated and triggers translesion DNA synthesis (TLS) by recruiting polymerase-eta. However, it remained unknown how error-prone TLS is turned off after DNA lesion bypass to prevent mutagenesis. Here we showed that ISG15 modification (ISGylation) of PCNA plays a key role in TLS termination. Upon UV irradiation, EFP, an ISG15 E3 ligase, bound to mono-ubiquitinated PCNA and promoted its ISGylation. ISGylated PCNA then tethered USP10 for deubiquitination and in turn the release of polymerase-h from PCNA. Eventually, PCNA was deISGylated by UBP43 for reloading of replicative DNA polymerases and resuming normal DNA replication. However, ISGylation-defective Lys-to-Arg mutations in PCNA or knockdown of any of ISG15, EFP, or USP10 led to persistent recruitment of mono-ubiquitinated PCNA and polymerase-eta to nuclear foci, causing an increase in mutation frequency. These findings establish a crucial role of PCNA ISGylation in termination of error-prone TLS for preventing excessive mutagenesis. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | 자연과학 | en |
dc.title | Modification of PCNA by ISG15 Plays a Crucial Role in Termination of Error-Prone Translesion DNA Synthesis | en |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 양승욱 | - |
dc.contributor.AlternativeAuthor | 유경륜 | - |
dc.contributor.AlternativeAuthor | 가승현 | - |
dc.contributor.AlternativeAuthor | 이성원 | - |
dc.contributor.AlternativeAuthor | 설재홍 | - |
dc.contributor.AlternativeAuthor | 전영주 | - |
dc.contributor.AlternativeAuthor | 정진하 | - |
dc.identifier.doi | 10.1016/j.molcel.2014.03.031 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2014-01/102/0000001279/1 | - |
dc.description.srnd | SEQ:1 | - |
dc.description.srnd | PERF_CD:SNU2014-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000001279 | - |
dc.description.srnd | ADJUST_YN:Y | - |
dc.description.srnd | EMP_ID:A004389 | - |
dc.description.srnd | DEPT_CD:3344 | - |
dc.description.srnd | CITE_RATE:14.464 | - |
dc.description.srnd | FILENAME:park et al (mc).pdf | - |
dc.description.srnd | DEPT_NM:생명과학부 | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2014-01/102/0000001279/1 | - |
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