비특이적인자가 거식세포활동에 미치는 영향에 관한 연구

Abstract

Various studies using the peritoneal macrophages of experimental animals have been reported to incrca se the population of the peritoneal macrophages by intraperitoneal injection of non-specific stimulants such as glycogen, mineral oil. newborn calf serum, thioglycollate broth. sodium casinale, etc. Recent re ports suggested that many fu r,ctional differences \\τrc expected bewcen the normal resident macrophagcs 2nd the macophages induced with rwn-specific stimulation However. there were few reports concerning the phagocytic activities of such stimulated macrophagcs on the living particles such as bacteria. An expcri ment was performed to understand the infltenecs of thioglycollate broth and fetal ealf sen:m as non-spe ific stimulants on the phagocytic activities of the mouse peri toneal macrophagcs Mice of DDO srain were divided into two groups which were injected intraperitoneal1y with 2.0ml of fluid thioglycollate medium and O.5ml of fetal calf serum. respectively. The peritoneal exudate cdls were collected 72 hours after thc injection of fluid thioglycollate mcdinm and 48 hours afler thc injection of fetal calf serum. The collected cells were pooled, suspended in the cell maintenance medium, and mixed with the suspension of St aphylococcus aUTeus(Wood-46) at 4°C. to make the bacteria-cell suspension. The bacteriacell suspensions were incubated at 37°C. for 30min. , 60min .• gOmin.• and 120min. , and each suspension was centrifuged (110g , for 5min.) after incubation The viable becteriaI units were counted from the supernatants. The pcrcentages of the viable bacterial units and the phagocytic indices at each incubation time were determined to express the degree of phagocytosis. At the same time , from the stained sediments, the ratios of the macrophages associated with bacteria to the total ones and the distribution of the macrophages laden with bacteria by the bacterial number were also checked. The results obtaincd in this experiments were summerized as follows; 1. The phagocytic indices of the thioglycollate stimulated macrophages were 1. 5 times higher than those of the unstimulated ones until 60 minutes' incubation. but thereafter, were not significantly diff· crent between them. The thioglycollate stimulated macrophages were associated with S. aureus 1. 5'""' 1. 8 times more than the unstimulated ones. and the uumbcrs of the bacteria within or attached to the thioglycollate stimulatcd macrophagcs were also remarkably greater than in case of the unstimulated ones during incubation. 2. No significant differences in the phagocytic indices. the ratios of the macrophages associated with baeleria, and the numbers of bacteria within or attached to the macrophages were observed betwecn the fetal calf serum stimulated macrophages and the uns' timulatcd ones during incubation. It, therefore, was concluded that the phagocytosis of Staphylococcus aureus by the mouse peritoneal rnacrophage was considerably enhanced with the treatment of fluid thioglycollate medium , whereas it was not altered with the treatment of fetal calf serum.