S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 19 No.3 (1978)
발생중인 태아 뇌하수체의 「호르몬」 분비세포에 관한 면역조직화학 및 전자현미경적 연구
Immunohistochemical and Electron Microscopic Studies on the Hormone Secretory Cells in the Developing Adenohypophysis the Human Fetuses
- 조사선; 이명복; 백상호
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.19 No.3, pp. 132-144
- The hypophyses of human fetuses aged from 5 to
40 weeks were studied with the peroxidase-laheled
antibody method and the electron microscope to investigate
hormone secretory cells , and to ascertain
the fine structural evidence for hormone synthesis.
The pituitary gland excised were fixed in Bouins
fluid and serial sections were made at 5p. Tissue
sections were reacted with rabbit antisera to human
growth hormone. prolactin. thyrotropin. and human
chorionic gonadotropin respectively and then with
peroxidase-labeled sheep anti-rabbit gamma globulin.
followed by enzyme histochemical reaction. Observation
were made under the light microscope. Some
tissues were fixed in glutaraldehyde and osmium
tetroxide. and stained with uranyl acetate and lead
citrate for electron microscopic observation.
The results observed were summarized as follows.
1. Somatotrophs were detected first at 10 weeks.
mammotrophs at 18 weeks , thyrotrophs and gonadotrophs
at 12 weeks respectively. but no hormone
secretory cells were identified before 10 weeks
2. Somatotrophs and mammotrophs were found throughout
the the pars distalis and most numerous in
the lateral regions. and gonadoptrophs and thyrotrophs
appeared chiefly in the posteromedian zone
and in its superior portion
3. Average numbers of hormone secretory cells and
reacting intensities of the cytoplasmic granules
generally increased with advancing fetal age.
4. Electron microscopic findings of secretory cells
were the presence of distinct granules, extensive
development of rough surfaced endoplasmic reti
culum and signs of granular formation in Golgi
complex. and these findings strongly suggest that
fetal adenohypophysis was producing hormones
during its development.