Browse

Acetylcholinesterase에 대한 유기인산제 농약의 억제작용과 Oxime에 의한 재활성작용에 관한 연구 : In Vitro Reactivation of Acetylcholinesterase Inhibited by Paration and PAP

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
서유헌홍사악
Issue Date
1978-12
Publisher
서울대학교 의과대학
Citation
Seoul J Med, Vol.19 No.4, pp. 143-149
Abstract
Cholinesterase in human blood cells and plasm'"
are inhibited in varying degrees by organophosphorus:
insecticides and nerve agents through phosphorylation
The phosphorylated enzymes can often be reactivated
by nucleophilic compounds such as oximes and
hydroxamic acids provided conversion of inhibited
enzyme to a nonreactive form(aging) has not occured
In this work. the effects of organophosphorus insecticides
(Parathion and PAP) on membrane-bound
acetylcholinesterase from human erythrocyte ghosts
were studied by using automatic recording spectrophotometer
according to the method of Ellman et aL Also, the influence of the oxime upon reactivation
was studied.
1. There was a concenturation dependent inhibition
<>f acetylcholinesterase by parathion and PAP. The
inhibition of acetylcholinesterase by PAP was shown
to be more rapid and complete than that by para
thion.
2. Decreasing the concenturation of 2-PAM below
정 x lO M resulted in incomplete reactivation. At
therapeutical concenturation of 2-PAM, 82.6% of the
enzyme activity was restored in parathion-inhibited
'enzyme , and 64.0% in PAP inhibited enzyme.
3. In vitro reactivation by 2-PAM of PAP inhibited
cholinesterase was shown to be more difficult than
that of paration-inhibited cholinesterase
4. Maximal reactivation of inhibited acetylcholinesterase
was noted 30 minutes after addiction of 2
-PAM and thereafter the degree of reactivation decTeased.
5. No spontaneous reactivation was noticed during
rthe time of the experiment
It was concluded that the rate and ease of inhibition
and reactivation are dependent on the bulk of the
side chain(leaving group) , and the phosphorylated
oximes may act as anticholinesterase.
ISSN
0582-6802
Language
Korean
URI
https://hdl.handle.net/10371/9362
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 19 No.4 (1978)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse