Detection of Avian Influenza-DNA Hybridization using a Wavelength Interrogation-based Surface Plasmon Resonance Biosensor

Abstract

A wavelength interrogation-based surface plasmon resonance (SPR) biosensor has been investigated for a detection of avian influenza DNA (AI-DNA). The immobilization of oligonucleotides and hybridization reaction of AI-DNA was monitored in real time without labeling. The results showed that SPR biosensor can provide linear detection suitable for quantification of AI-DNA. This study demonstrates the potential for a simple AI virus diagnosis. Since these viruses can be transmitted into humans and often are fatal, improved AI detection is important.