Freund's Adjuvants 및 그 조성인자의 혈관증식작용

Abstract

The original Freunds Adjuvant is a water-in-paraffin- oil emulsion, which has been used for the purpose of enhancing antibody production; but the adjuvant combined with killed mycobacterium is generally called Freunds adjuvants (F.A.). In 1960 Dale reported first of the lymphatics proIiferation effect of F.A. in mice, but he did not elucidate whether such effect is due to the complete F.A. or to each of its components. This study was done to confirm the vascularization effect of F.A. and to determine if the effect is based upon the complete F.A. or upon its components. Fifty-five adult albino rabbits were injected respectively subcutaneously (ear), subserously (stomach) and intramuscularly (buttock) with O. 1 C.c. of complete F.A. and its components; and the site of each injection was examined for the subsequent period by simple inspection , tranillumination and biopsy. The results are as follows. 1) Complete F. A., when injected subcutaneously, produced marked proliferation of blood vessels both macroscopically and microscopically at the site of injection. Such effect of this agent reached its maximum peak on approximately one month after injection and lasted at least 3 months. 2) The oil fraction of the adjuvant (original F.A.) produced nearly the same degree of vascularization as the complete F.A. , whereas each component of the adjuvant showed no or little response. 3) It would appear therefore that the vascularization effect of F.A. is mainly due to its oil fraction and this is probably based on the synergy of paraffin-oil and lanolin 4) F.A. and its components , when injected subser ously, showed little vascularization, but not any at the site of intramusclar injection.