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Guanine Aminohydrolase의 활성분포
Activity Distribution of Guanine Aminohydrolase in Rat Tissues

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Authors
김승원; 김정자; 박주배
Issue Date
1981-03
Publisher
서울대학교 의과대학
Citation
Seoul J Med, Vol.22 No.1, pp. 34-40
Abstract
Enzyme activities are the most frequent examples
of tissue components used in characterizing
tissue functions; and standardization of relative
enzyme activities, therefore, among tissues in
health and disease has relevance of biochemical
studies to particular biological problems of any
kind.
The bias in favor of enzymes with this regards,
however, seems critical in any case of
enzyme because the size of the rate units of
enzymes is mostly determined by various degrees
of subtleties of the reaction conditions as used
by each reporter (Knox, 1976). It seems not
to be exceptional in the case of guanine aminohydrolase
(EC 3. 5. 4. 3) , while it exerts an
important r91e in purine catabolism which not
only involves interconversions between purines
at the level of the monophosphates, the ribosides
, and the free bases , but also gives rise to
uric acid as the end product in man and other
primates as well as in birds and terrestial
reptiles and to allantoin in mammals other than
the primates (Kimm, 1973).
The enzyme has been under continuous investigation
during the last several years by the
authors' laboratory which has proved its isoenzymic
nature (Kimm et at. , 1967: Cha et at. ,
1980) , its significances in health and disease
(Kimm and Lee, 1970; Kimm and Han , 1971),
proposing a new colorimetric method for the
determination of its activity (Park and Kimm,
• Present Address: Department of Chemistry,
Cnllege of Natural Sciences, Dong-A University
1976) and a staining method for it on the electrophoretic
gel bed (Kimm , 1976).
During this period it attracted the authors'
attention that observations on activity patterns
of this enzyme among tissues were so scanty
and inconsistent to compare for the purpose of
characterizing tissue functions pertinent to this
particular enzyme, not to mention little progress
made in the purification of the enzyme
since the initial works (Schmidt, 1932: Kalckar,
1947) as well as in the study of its properties
(Kumar et at. , 1965: Currie. , 1967: Kumar et
at. , 1973)
Fostered by interest in the regulation of
purine catabolism, it came into the authors'
consideration to undertake analyses of activities
of guanine aminohydrolase distributed among
tissues of rats because we thought it to be a
burgeoning trial prior to any of the further
investigation on this enzyme, of which clinical
application, for instance, would cast a clue to
pathological tissue diagnoses together with its
biochemical implications regarding purine catabolism
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/9474
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 22 No.1 (1981)
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