S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 22 No.2 (1981)
급성 일산화탄소중독이 백서간장에 미치는 영향에 관한 병리조직학적 및 효소조직화학적 연구
Histopathologic and Enzyme Histochemical Studies on Effects of Acute Carbon Monoxide Poisoning in Rat Liver
- 함의근; 송계용; 윤덕로
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.22 No.2, pp. 189-198
- An experimental study was performed to observe
the acute carbon monoxide poisoning in the liver of
rats by means of histopathologic and enzyme histoch
emical methods, attempting to explore the mechanism
behind the process of the acute anoxic necrosis of
Experimental animals(65 Sprague-Dawley rats , 210
~280gm_) were devided into four groups by the
duration of exposure and the concentration of carbon
monoxide: Control (15 rats , unexposures) , 1% CO
exp.(30 rats, 30 min.) , 0.7% CO exp. (12 rats,60
min.) and 0.4% CO expo (8 rats , 120 mip.). Histop
hological investigation and the enzyme activities of
succinic dehydrogenase (SDH) , NADH diaphorase,
lactic dehydrogenase (LDH) , alkaline phosphatase
(alk. P.) and acid phosphatase(acid P.) in liver were
observed by hematoxylin and eosin staining and
appropriate enzyme histochemical stainings.
The results obtained were as follows:
1. Histologic sections of rat liver in groups of
acute poisoning with carbon monoxide revealed mild
to moderate congestion of central vein and sinusoidal
spaces, accompanied by acidophilic degeneration of
hepatocytes, pyknosis, fatty changes and focal necrosisy.
Central zone was more severely affected than
portal zone and the reactions were more marked in
higherconcentration and shott duration groups.
2. SDH and NADH diaphorase activities in all
experimental group were significantly decreased in
mild to moderate degrees, especially in central and
3. LDH activities in all experimental rousps generally
decreased in mild degree in the central and
midzone in contrast to the increase in mild to moderate
degree of portal zone.
4. Changes of alk. P. activities were not noticed.
Acid phosphatase activities in all experimental groups
were significantly incre-ased in 60 to 120 min. expo
5. Above histopathologic and histochemical findings
seemed to support that acute anoxic necrosis of liver
could be included by acute carbon monoxide poisoning
preceded by hepatic enzymeatic changes and the most
vulnerable portion of hepatic parenchyme was to be
central zone of hepatic lobule.