연층 백혈구 응집반응의 기전

Abstract

aggregation (BCLA) reaction was the simple and sensitive test for cell-mediated immunity. Thereafter, some clinical approaches were performed by several investigators. However the mechanism of human BCLA reaction was not studied in detail , This study was designed to clarify some factors involved in the mechanism of BCLA reaction and results are summarized as follows. 1. The strong correlation was observed between minimal amounts of PPD required for BCLA reaction and skin indura tion diameter of tuberculin reaction (correlation coefficiency=-0.648, p<0.005)) 2. Optimal temperature of BCLA reaction was 37'C and reaction time was 4 to 6 hours 3. Calcium ion was essential for BCLA reaction 4. Cellular composition of aggregates was mostly of polymorphonuclear leukocytes and partly of Iymphocytes and monocytes. 5. BCLA reaction appeared to be mediated by solublefactor released from sensitized lymphocyte.