희석산증때의 골격근의 세포내 pH

Abstract

Hydrochloric acid and various extracellular fluid expanding solutions were administered to ten dogs and extracellular and intracellular acid-base changes were studied. Intracellular pH was determined by distribution of 5, 5-dimethyl-2, 4-oxazolidinedione. The following data were obtained. l. Extracellular hydrogen ion concentration of dogs anesthetized with Nembutal was 41±4.0 nM/L and pH averaged 7.30. Intracellular hydrogen ion concentration of skeletal muscle was I06±4.0 nM/L and intracellur pH averaged 6.98. Intracellular and extracellular bicarbonate ion concentration were 10.1± 0.5 mEq/L and 25±O.8 mEq/L. respectively. There fore hydrogen ion concentration ratio inside to outside CHi/He) ￦a s 2.59 and potassium ion concentration ratio CKi/Ke) was 35. 5. 2. Hydrochloric acid was infused 5 mM/kg body weight to 3 dogs. In Experiment I and 2, after the administration of hydrochloric acid , extracellular pH decreased from 7.38 to 7.13 and from 7.39 to 7.16. However, intracellular pH was maintained relatively constant from 6.99 to 6.96 and from 7.00 to 6.95. In experiment 3, extracellular pH decreased more than in experiment I and 2, from 7.39 to 6.98 and intracellular pH decreased from 6.98 to 6.82. It was noticed that during moderate acute acidosis, intracellular pH will not always change as to be expected 3. In experiment 4 to experiment 10, various extracellular fluid expanding solutions were administered approximately 25 ml/kg body weight for two hours and acid-base data were monitored. There were no remarkable changes in acid-base parameters after the infusion of isotonic saline, isotonic balanced elee trolyte solution, and isotonic mannitol. Infusion vele> city was about twice as much as practical use in clinic. It was clear that dilution acidosis is not to be induced with this rate of infusion velocity. When hypertonic solutions were infused in experiment 5 and 10, there were eminent increases of eλtracellular potassium ion concentration. There might be solvent drag effect in this process. At the level of 23 ml/kg for two hours infusion with extracellular expanding solution, buffering by body buffers will well tolerate to this level of bicarbonate dilution. However, when hypertonic solution was infused, extracellular pota ssium ion concentration might be rapidly increased