Testosterone의 방사면역측정법의 정립과 한국인 아동 및 정상 성인의 혈청내 농도

Abstract

In order to improve a simple and reliable redioim' munoassay method for the determination of testoste' rone in human serum, various factors affecting the assay quality were investigated: the purity of tracer; ionic strength; pH and steroid carriers of buffer sol· ution; and the contact time and temperature of charcoal with antigen'antibody complex etc. The present method was evaluatcd in terms of accuracy, precision and the results of internal and external quality contro l. The concentrations (mean±SD) of testosterone in serum from 136 healthy normal men(aged 15~50yr) and from 116 prepubertal boys(aged 7~13yr) were 6. 85±2. 05ng/ml and O. 19±0.13, respectively. There were no significant changes in the serum levels as the age was increased in each group. The concentration from 35 normal ovulatory women was 420± 70pg/ml. These values were compared with the results of other investigators and those from several commercial kits.