인삼 성분이 oxygen radical의 피부 collagen gelation에 대한 작용에 미치는 영향

Abstract

most types of cellular macromolecules. These radicals can be formed as products from altered cellular metabolism in certain pathologic states or by radiation energy. Consequently, their destructive action to many biological compounds are suggested to play roles in tissue damage under various pathologic conditions including aging phenomenon Cutaneous tissue may be one of the tissues that are constantly subject to attack of the oxygen radicals because it has more chances to be exposed to Iadia tion than others. Recently, degradative action of oxygen radicals have been demonstrated on collagen and proteoglycan which are major components of skin tissue. Thus, the oxygen radicals in this tissue are suggested to participate in aging as well as other degradative changes. In view of the recent reports showing that ginseng components have antioxidant effect and quenching action to an organic radical, it is of interest to see effects of ginseng components on oxidative process by oxygen radicals to assess their actions with regard to their protective effects on tissue damage In the present study, effects of ginseng components, were observed on inhibition of gelation of skin collagen by oxygen radicals generated by xanthine and xanthine oxidase, and quenching effects of the components on each oxygen radical were also observed to elucidate their mechanism of antioxidant effect observed in the present study. The results were as follows. 1. Collagen gelation was inhibited by treatment with xanthine and xanthine oxidase 2. The inhibited gelation was recovered by total extract of ginseng. The protective effect on gelation was observed with nonsaponin fraction , not with saponin fraction 3. But collagen gelation and xanthine oxidase by themselves were not affected by ginseng components used. 4. Among the oxygen radicals suggested to be produced in the xanthine oxidase system, total extract showed quenching action to 'a" although the action was not so remarkable as that of other 102 quenchers. This effect was observed with nonsaponin fraction The results suggest that ginseng components have protective effect on collagen alteration induced by oxygen radicals and this effect may be attributed to 102 quenching action of nonsaponin fraction of gmseng