Browse

Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity

Cited 102 time in Web of Science Cited 100 time in Scopus
Authors
Hwang, Injae; Park, Yoon Jeong; Kim, Yeon-Ran; Kim, Yo Na; Ka, Sojeong; Lee, Ho Young; Seong, Je Kyung; Seok, Yeong-Jae; Kim, Jae Bum
Issue Date
2015
Publisher
Federation of American Society of Experimental Biology
Citation
The FASEB Journal, vol.29 no.6, pp. 2397-2411
Keywords
gut hormoneinsulin sensitivitymetabolites
Abstract
Firmicutes and Bacteroidetes, 2 major phyla of gut microbiota, are involved in lipid and bile acid metabolism to maintain systemic energy homeostasis in host. Recently, accumulating evidence has suggested that dietary changes promptly induce the alteration of abundance of both Firmicutes and Bacteroidetes in obesity and its related metabolic diseases. Nevertheless, the metabolic roles of Firmicutes and Bacteroidetes on such disease states remain unclear. The aim of this study was to determine the effects of antibiotic-induced depletion of Firmicutes and Bacteroidetes on dysregulation of energy homeostasis in obesity. Treatment of C57BL/6J mice with the antibiotics (vancomycin [V] and bacitracin [B]), in the drinking water, before diet-induced obesity (DIO) greatly decreased both Firmicutes and Bacteroidetes in the gut as revealed by pyrosequencing of the microbial 16S rRNA gene. Concomitantly, systemic glucose intolerance, hyperinsulinemia, and insulin resistance in DIO were ameliorated via augmentation of GLP-1 secretion (active form; 2.03-fold, total form; 5.09-fold) independently of obesity as compared with untreated DIO controls. Furthermore, there were increases in metabolically beneficial metabolites derived from the gut. Together, our data suggest that Firmicutes and Bacteroidetes potentially mediate insulin resistance through modulation of GLP-1 secretion in obesity.—Hwang, I., Park, Y. J., Kim, Y. -R., Kim,
Y.N.,Ka, S., Lee,H.Y., Seong, J.K., Seok,Y. -J., andKim, J.B. Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity.
Language
English
URI
https://hdl.handle.net/10371/95461
DOI
https://doi.org/10.1096/fj.14-265983
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse