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Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy

Cited 136 time in Web of Science Cited 146 time in Scopus
Authors
Kim, Deok-Ho; Seo, Chang-Ho; Han, Karam; Kwon, Keon Woo; Levchenko, Andre; Suh, Kahp Yang
Issue Date
2009-05-22
Publisher
Wiley-Blackwell
Citation
Adv. Funct. Mater. 2009, 19, 1579
Keywords
cell migrationgradientsmicropatternstopography
Abstract
This work reports the design of and experimentation with a topographically patterned cell culture substrate of variable local density and anisotropy as a facile and efficient platform to guide the organization and migration of cells in spatially desirable patterns. Using UV-assisted capillary force lithography, an optically transparent microstructured layer of a UV curable poly(urethane acrylate) resin is fabricated and employed as a cell-culture substrate after coating with fibronectin. With variable local pattern density and anisotropy present in a single cell-culture substrate, the differential polarization of cell morphology and movement in a single experiment is quantitatively characterized. It is found that cell shape and velocity are exquisitely sensitive to variation in the local anisotropy of the two-dimensional rectangular lattice arrays, with cell elongation and speed decreasing on symmetric lattice patterns. It is also found that cells could integrate orthogonal spatial cues when determining the direction of cell orientation and movement. Furthermore, cells preferentially migrate toward the topographically denser areas from sparser ones. Consistent with these results, it is demonstrated that systematic variation of local densities of rectangular lattice arrays enable a planar assembly of cells into a specified location. It is envisioned that lithographically defined substrates of variable local density and anisotropy not only provide a new route to tailoring the cell-material interface but could serve as a template for advanced tissue engineering.
ISSN
1616-301X
Language
English
URI
http://hdl.handle.net/10371/9547
DOI
https://doi.org/10.1002/adfm.200801174
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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