Stepwise self-assembly of a protein nanoarray from a nanoimprinted poly(ethylene glycol) hydrogel

Abstract

Ultraviolet nanoimprint lithography is used to fabricate nanopatterns of poly(ethylene glycol) (PEG) hydrogel on gold substrates for the construction of protein nanoarrays. A protein nanoarray with a minimum feature size of 100 nm is constructed in the nanowell of the PEG hydrogel by self-assembly with a biotinylated PEG-disulfide, streptavidin, and biotinylated antibody, sequentially (see figure), and confirmed by varying the nanowell height between each step in both solution and air.