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Isolation and comparative genomic analysis of T1-Like Shigella bacteriophage pSf-2
Cited 17 time in
Web of Science
Cited 17 time in Scopus
- Authors
- Issue Date
- 2016-03
- Publisher
- Springer Verlag
- Citation
- Current Microbiology, Vol.72 No.3, pp.235-241
- Abstract
- The increasing prevalence of antibiotic-resistant Shigella sp. emphasizes that alternatives to conventional antibiotics are needed. Siphoviridae bacteriophage (phage), pSf-2, infecting S. flexneri ATCC (R) 12022 was isolated from Geolpocheon stream in Korea. Morphological analysis by transmission electron microscopy revealed that pSf-2 has a head of about 57 +/- 4 nm in diameter with a long tail of 136 +/- 3 nm in length and 15 +/- 2 nm in width. One-step growth analysis revealed that pSf-2 has latent period of 30 min and burst size of 16 PFU/infected cell. The DNA genome of pSf-2 is composed of 50,109 bp with a G+C content of 45.44 %. The genome encodes 83 putative ORFs, 19 putative promoters, and 23 transcriptional terminator regions. Genome sequence analysis of pSf-2 and comparative analysis with the homologous T1-like Shigella phages, Shfl1 and pSf-1, revealed that pSf-2 is a novel T1-like Shigella phage. These results showed that pSf-2 might have a high potential as a biocontrol agent to control shigellosis. Also, the genomic information may lead to further understanding of phage biodiversity, especially T1-like phages.
- ISSN
- 0343-8651
- Language
- English
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