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In vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNA

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dc.contributor.authorJeon, Yong Hyun-
dc.contributor.authorChoi, Yun-
dc.contributor.authorKang, Joo Hyun-
dc.contributor.authorChung, June-Key-
dc.contributor.authorLee, Yong Jin-
dc.contributor.authorKim, Chul Woo-
dc.contributor.authorJeong, Jae Min-
dc.contributor.authorLee, Dong Soo-
dc.contributor.authorLee, Myung Chul-
dc.date.accessioned2009-09-24T23:59:12Z-
dc.date.available2009-09-24T23:59:12Z-
dc.date.issued2006-01-30-
dc.identifier.citationVaccine 2006;24(16):3057-62en
dc.identifier.issn0264-410X-
dc.identifier.urihttps://hdl.handle.net/10371/9803-
dc.description.abstractThe administration of naked DNA into animals is increasing as a research tool to develop DNA vaccine. To monitor the distribution and duration of gene expression of a DNA vaccine in living organisms, we used the naked DNA encoding firefly luciferase (Fluc) as an imaging reporter gene, and evaluated in vivo bioluminescent images in a murine model. We observed bioluminescence at the injection site and at inguinal lymph node from 10h to 24h post-injection when DNA vaccine encoding Fluc (pcDNA3.1-Fluc) was injected into the bilateral posterior flanks in mice. Fluc gene expressions at injection sites and unilateral posterior flank inguinal lymph node were also confirmed by RT-PCR. However, when pcDNA3.1-Fluc was injected into the mid-dorsum bioluminescent signals were observed at the injection site for up to 14 days post-injection, but no bioluminescent signals were detected in inguinal lymph nodes. Concurrent mRNA expressions of Fluc gene at injection sites but not at inguinal lymph nodes were confirmed by RT-PCR. These findings suggest that optical imaging using Fluc could be useful for monitoring the location, intensity and duration of gene expression of naked DNA vaccines in living animals non-invasively and repetitively.en
dc.description.sponsorshipThis work was supported in part by grants from The
Cancer Research Center, the Korean Science & Engineering
Foundation (KOSEF) through the Tumor Immunity Medical
Research Center at Seoul National University College of
Medicine, Korea. Y.H. Jeon, J.H. Kang, Y.J. Lee, and Y. Choi
were supported by the BK21 Project for Medicine, Dentistry,
and Pharmacy in Korea (2004).
en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectFirefly luciferaseen
dc.subjectIn vivo visualizationen
dc.subjectDNA vaccine gene expressionen
dc.titleIn vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNAen
dc.typeArticleen
dc.contributor.AlternativeAuthor전용현-
dc.contributor.AlternativeAuthor최윤-
dc.contributor.AlternativeAuthor강주현-
dc.contributor.AlternativeAuthor정준기-
dc.contributor.AlternativeAuthor이용진-
dc.contributor.AlternativeAuthor김철우-
dc.contributor.AlternativeAuthor정재민-
dc.contributor.AlternativeAuthor이동수-
dc.contributor.AlternativeAuthor이명철-
dc.identifier.doi10.1016/j.vaccine.2006.01.033-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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