근육 유지에 관여하는 E3 유비퀴틴 라이게이스인 Mind Bomb-1의 기능 연구

Abstract

Maintenance of skeletal muscle throughout life is essential for quality of life. Hence, it is of great interest to determine the muscle-specific factor responsible to maintain skeletal muscle throughout the lifetime. In skeletal muscle, several muscle-specific E3 ubiquitin ligases are known to play fundamental roles in maintenance of muscle, regulation of muscle protein catabolism and mediation of muscle atrophy. Among the great diversity of E3 ubiquitin ligases, however, there are no studies in muscle on the E3 ubiquitin ligase Mind bomb 1 (Mib1). In this study, I used Cre-Lox targeted approach to establish a myofiber-specific Mib1 knockout mice to determine whether myofiber-specific Mib1 deletion compromises muscle maintenance contributing to progressive muscle atrophy. Here, I first confirmed that the genetic lack of myofiber-specific Mib1 leads to loss of muscle mass, decreased cross-sectional area and impaired muscle functions in adult mice. These findings suggest Mib1 may have potential role to prevent skeletal muscle atrophy.