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Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes

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Authors
Shin, Daehyun; Lee, Yoonjin; Huang, Yu-Hua; Lim, Hye-Won; Jang, Kyounghee; Kim, Dae-Duk; Lim, Chang-Jin
Issue Date
2018-06-26
Citation
BMC Complementary and Alternative Medicine, 18(1):196
Keywords
Agastache rugosaAnti-photoagingGlutathioneHaCaTMatrix metalloproteinase (MMP)Probiotic fermentationReactive oxygen species (ROS)Superoxide dismutase
Abstract
Background
Agastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-induced photooxidative stress. The present work aimed to examine whether probiotic bacterial fermentation would potentiate the skin anti-photoaging activity of ARE or not, by comparing the protective properties of ARE and corresponding fermented extract (ARE-F) against UV-B radiation-induced photooxidative stress in HaCaT keratinocytes.

Methods
ARE-F was produced from ARE by the fermentation with Lactobacillus rhamnosus HK-9, a type of Gram-positive probiotic bacterial strain. Anti-photoaging activities were evaluated by analyzing reactive oxygen species (ROS), promatrix metalloproteinases (proMMPs), total glutathione (GSH) and total superoxide dismutase (SOD) in UV-B-irradiated HaCaT keratinocytes. Antiradical activity was determined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay.

Results
ARE-F contained higher attenuating activity on the UV-B-induced ROS generation than ARE. Similarly, ARE-F was able to diminish the UV-B-induced proMMP-9 and -2 more effectively than ARE. ARE-F displayed higher tendencies to augment the UV-B-reduced total GSH content and SOD activity than ARE. However, there were no significant difference between ARE and ARE-F in ABTS radical scavenging activities.

Conclusions
The findings suggest that the UV-B radiation-protective activity of ARE is enhanced by probiotic bacterial fermentation, which might improve the therapeutic and cosmetic values of A. rugosa leaves.
ISSN
1472-6882
Language
English
URI
http://hdl.handle.net/10371/142837
DOI
https://doi.org/10.1186/s12906-018-2194-9
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College of Pharmacy (약학대학)Research Institute of Pharmaceutical Sciences (종합약학연구소)Journal Papers (저널논문_종합약학연구소)
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