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RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
Cited 35 time in
Web of Science
Cited 38 time in Scopus
- Authors
- Issue Date
- 2014-07
- Publisher
- Genetics Society of America
- Citation
- G3: Genes, Genomes, Genetics, Vol.4 No.7, pp.1291-1295
- Abstract
- We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
- ISSN
- 2160-1836
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