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dCas9-mediated nanoelectrokinetic direct detection of target gene for liquid biopsy

Cited 9 time in Web of Science Cited 14 time in Scopus
Authors
Lee, Hyomin; Choi, Jihye; Jeong, Euihwan; Baek, Seongho; Kim, Hee Chan; Chae, Jong-Hee; Koh, Youngil; Seo, Sang Woo; Kim, Jin-Soo; Kim, Sung Jae
Issue Date
2018-12
Citation
Nano Letters, Vol.18 No.12, pp.7642-7650
Keywords
Ion concentration polarizationselective preconcentrationdCas9liquid biopsymicro/nanofluidicsdirect detection
Abstract
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics.
ISSN
1530-6984
URI
http://hdl.handle.net/10371/165693
DOI
https://doi.org/10.1021/acs.nanolett.8b03224
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