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Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
Cited 23 time in
Web of Science
Cited 23 time in Scopus
- Authors
- Issue Date
- 2021-06-03
- Publisher
- BMC
- Citation
- Genome Biology. 2021 Jun 03;22(1):170
- Keywords
- Prime editor ; Igf2 ; Adamts20 ; Mouse cells and embryos ; Germline transmission ; Dwarf phenotype ; Proximal dead sgRNA ; Chromatin-modulating peptides
- Abstract
- Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.
- ISSN
- 1474-760X
- Language
- English
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