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Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration
Cited 119 time in
Web of Science
Cited 128 time in Scopus
- Authors
- Issue Date
- 2007-11-02
- Publisher
- Springer Verlag
- Citation
- Biotechnol Lett. 2008 Mar;30(3):405-10. Epub 2007 Nov 1.
- Keywords
- Biocompatible Materials ; Cell Adhesion ; Cell Line ; Cell Proliferation ; Fibroins/*chemistry ; Microscopy, Electron, Scanning ; Osteoblasts/*cytology/physiology/ultrastructure ; Osteogenesis ; Porosity ; Tissue Engineering/*methods ; Bone Regeneration ; Nanostructures ; Tissue Scaffolds
- Abstract
- We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
- ISSN
- 0141-5492 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17973083
https://hdl.handle.net/10371/23985
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