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Detection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencing

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Authors
Yun, Yeo-Jun; Lee, Keun-Hwa; Haihua, Lin; Ryu, Yoon-Jong; Kim, Bum-Joon; Lee, Young-Ho; Baek, Goo-Hyun; Kim, Hee-Joong; Chung, Moon-Sang; Lee, Myung-Chul; Lee, Sang-Hoon; Choi, In-Ho; Cho, Tae-Jun; Chang, Bong-Soon; Kook, Yoon-Hoh
Issue Date
2005-01-07
Publisher
American Society for Microbiology
Citation
J Clin Microbiol. 2005 Jan;43(1):174-8.
Keywords
Antitubercular Agents/pharmacologyBiopsyCloning, MolecularDNA-Directed RNA Polymerases/*geneticsHumansJoints/*microbiologyMycobacterium tuberculosis/*classification/drugeffects/genetics/*isolation & purificationPlasmidsPolymerase Chain Reaction/*methodsRifampin/pharmacologySequence Analysis, DNATuberculosis, Osteoarticular/*microbiology
Abstract
Osteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an rpoB PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the rpoB sequences determined with those in GenBank, Mycobacterium tuberculosis was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant M. tuberculosis strains. Our results suggest that the rpoB PCR-TA cloning-sequencing method developed, which detects M. tuberculosis and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.
ISSN
0095-1137 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15634968

http://hdl.handle.net/10371/29596
DOI
https://doi.org/10.1128/JCM.43.1.174-178.2005
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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