SHERP

TGF-h1-mediated activations of c-Src and Rac1 modulate levels of cyclins

Cited 0 time in webofscience Cited 27 time in scopus
Authors
Lee, Jung Weon; Kim, Hwang-Phill; Kim, Tai-Young; Lee, Mi-Sook; Jong, Hyun-Soon; Kim, Tae-You; Bang, Yung-Jue
Issue Date
2005
Publisher
Elsevier
Citation
Biochim. Biophys. Acta 1743 (2005) 151– 161
Keywords
Integrin; TGF-h1; Cyclin; Signal cross-talk; c-Src; Rac1
Abstract
Integrin-mediated cell adhesion transduces signals to regulate actin cytoskeleton and cell proliferation. While understanding how integrin
signals cross-talk with the TGF-h1 pathways, we observed lamellipodia formation and cyclin regulation in Hep3B cells, following TGF-h1
treatment. To answer if integrin signaling via actin organization might regulate cell cycle progression after TGF-h1 treatment, we analyzed
cross-talk between the two receptor-mediated pathways in hepatoma cells on specific ECMs. We found that basal and TGF-h1-mediated
activation of c-Src and Rac1, expression of cyclins E and A, and suppression of p27Kip1 were significant in cells replated on fibronectin, but
not in cells on collagen I, indicating a different integrin-mediated cellular response to TGF-h1 treatment. Levels of tyrosine phosphorylation
and actin-enriched lamellipodia on fibronectin were also more prominent than in cells on collagen I. Studies using pharmacological inhibitors
or transient transfections revealed that the preferential TGF-h1 effects in cells on fibronectin required c-Src family kinase activity. These
observations suggest that a specific cross-talk between TGF-h1 and fibronectin-binding integrin signal pathways leads to the activation of c-
Src/Rac1/actin-organization, leading to changes in cell cycle regulator levels in hepatoma cells. Therefore, this study represents another
mechanism to regulate cell cycle regulators when integrin signaling is collaborative with TGF-h1 pathways.
ISSN
0167-4889
Language
English
URI
http://www.elsevier.com/locate/bba
http://hdl.handle.net/10371/3240
DOI
https://doi.org/10.1016/j.bbamcr.2004.09.014
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse