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Semiquantitative Measurement of Murine Bleomycin-Induced Lung Fibrosis in In Vivo and Postmortem Conditions Using Microcomputed Tomography: Correlation With Pathologic Scores--Initial Results

Cited 21 time in Web of Science Cited 21 time in Scopus
Authors
Lee, Hyun Ju; Goo, Jin Mo; Kim, Na Ra; Kim, Min A; Chung, Doo Hyun; Son, Kyu-Ri; Kim, Hyo-Cheol; Lee, Chang Hyun; Park, Chang Min; Chun, Eun Ju; Im, Jung-Gi
Issue Date
2008-05-23
Publisher
Lippincott, Williams & Wilkins
Citation
Invest Radiol. 2008;43:453-460
Keywords
microcomputed tomographybleomycin, fibrosislungmurine
Abstract
To evaluate whether the semiquantification of lung inflammation and fibrosis in murine bleomycin-induced lung fibrosis using micro-computed tomography (micro-CT) in in vivo and postmortem conditions is feasible, and to correlate micro-CT and pathologic scores. MATERIALS AND METHODS: Bleomycin-induced lung fibrosis was created by intratracheally instilling 3 mg/kg of bleomycin into C57BL/6 mice. Mice were allocated randomly to 2-week, 4-week, and 8-week follow-up groups. In each group, in vivo and follow-up postmortem micro-CT were performed using a voxel size of 35 x 35 x 35 microm. Ground-glass opacity (GGO), consolidation, parenchymal lines, honeycombing, and peripheral bronchial dilatation were scored on micro-CT images in a semiquantitative fashion, whereas inflammation and fibrosis were scored histopathologically. The confidence levels of micro-CT findings were also scored. Correlations between micro-CT and pathologic findings were examined using Spearman rank correlation analysis, and differences between CT scores and confidence levels for in vivo and postmortem micro-CT were subjected to Wilcoxon signed rank testing. Agreements between in vivo and postmortem micro-CT scores were tested using weighted kappa statistics. RESULTS: Consolidation in vivo (r = 0.46) and at postmortem (r = 0.39) and GGO in vivo (r = 0.31) by micro-CT showed fair to moderate correlation with pathologic inflammation scores (P < 0.001). By in vivo and postmortem micro-CT, parenchymal lines (r = 0.72 vs. 0.83) showed good to excellent and peripheral bronchial dilatation (r = 0.47 vs. 0.68) showed moderate to good correlation with pathologic fibrosis scores (P < 0.001). For GGO, consolidation, peripheral bronchial dilatation, and parenchymal lines, fair to moderate agreement was obtained between in vivo and postmortem micro-CT. However, confidence levels for peripheral bronchial dilatation, parenchymal lines, and honeycombing were significantly higher by postmortem micro-CT (P < 0.001). CONCLUSIONS: Micro-CT scores and pathologic scores were found to be well correlated by in vivo and postmortem micro-CT. Although agreements between in vivo and postmortem micro-CT were significant, the confidence levels for fibrosis-related CT findings were significantly higher by postmortem micro-CT.
ISSN
0020-9996 (print)
1536-0210 (online)
Language
English
URI
http://hdl.handle.net/10371/4232
DOI
https://doi.org/10.1097/RLI.0b013e31816900ec

https://doi.org/10.1097/RLI.0b013e31816900ec
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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