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PCR Amplification of DNA Extracted from Paraffin-Embedded Tissue

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Authors
Lee, Jung Bin; Lee, Yoon Seong
Issue Date
1992-06
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1992;33(2):121-125
Keywords
PCRParaffin-embedded tissue(PET)Ether
Abstract
The polymerase chain reaction(PCR) DNA amplification method is a
powerful new tool in the field of individual identification or examination of medicolegal
evidences. Usually DNA is extracted from fresh tissue, blood, semen or dried specimen.
We try to find suitable way to obtain DNA from paraffin-embedded tissue(PET), the
most common preparation in pathological archives. Since PET processing conditions
vary in their suitability for amplification, variable methods l)simple long incubation 2)
xylene method 3)heating method 4)ether method were tried. Two VNTR loci of 017530
and apolipoprotein B gene were used for PCR amplification. The deparaffination process
using ether shows the best result in the DNA extraction and PCR amplication.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/6347
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 33 No.2 (1992)
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