Browse

Ischemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage

Cited 15 time in Web of Science Cited 14 time in Scopus
Authors
Hwang, In Koo; Yoo, Ki-Yeon; Kim, Dae Won; Kim, So Young; Park, Jun Hong; Ryoo, Zae Young; Kim, Joon; Choi, Soo Young; Won, Moo-Ho
Issue Date
2008-01-25
Publisher
Wiley-Blackwell
Citation
J Neurosci Res. 86(8):1823-1835
Keywords
AnimalsBase SequenceBrain Ischemia/genetics/*metabolism/*prevention & controlGene Expression Regulation/*physiologyGerbillinaeHumansMaleMiceMice, Inbred BALB CMolecular Sequence DataNeuroprotective Agents/*metabolismRibosomal Proteins/*biosynthesis/genetics
Abstract
Some ribosomal proteins are important regulators of development and DNA repair. However, few studies have been conducted on ribosomal protein S3 (rpS3) in the ischemic hippocampus. In the present study, we investigated ischemia-induced changes in rpS3 immunoreactivity, rpS3 mRNA, and protein levels in the hippocampal CA1 region of Mongolian gerbil after 5 min of transient forebrain ischemia. RpS3 immunoreactivity and its protein level were found to be significantly elevated at 6 hr after ischemia/reperfusion and then continuously decreased with time. RT-PCR analysis also showed that rpS3 mRNA levels were significantly elevated in CA1 at 6 hr after transient ischemia. In addition, during the course of this study, we developed a delivery vector (Pep-1) and its rpS3 fusion protein (Pep-1-rpS3) to elucidate the role of rpS3 in ischemia-induced damage. Pep-1-rpS3 administration to ischemic animals significantly and dose dependently increased neuronal survival in the stratum pyramidale of CA1. Moreover, Pep-1-rpS3 treatment reduced terminal deoxynucleotidyl dUTP nick-end labeling-positive CA1 pyramidal cell numbers in the stratum pyramidale. To elucidate how Pep-1-rpS3 ameliorates ischemic damage, changes in 4-hydroxy-2-nonnenal (HNE; an indicator of lipid peroxidation) immunoreactivity and protein levels were investigated. HNE levels and immunoreactivities in Pep-1-rpS3-treated ischemic animals were lower than in corresponding Pep-1-treated ischemic animals. These results indicate that rpS3 has a neuroprotective effect in the brain exposed to ischemia.
ISSN
1097-4547 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18214992

http://hdl.handle.net/10371/68168
DOI
https://doi.org/10.1002/jnr.21621
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse