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Increace in Intracellular Calcium is Necessary for RANKL Induction by High Extrecellular Calcium

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Authors
Kim, Gwan-Shik; Jun, Ji-Hae; Kim, Hyung-Keun; Woo, Kyung-Mi; Baek, Jeong-Hwa
Issue Date
2005-03
Publisher
Korean Acadamy of Oral Biology
Citation
International Journal of Oral Biology 30:9-15
Keywords
high extracellular calciumRANKLp44/42 MAPKintracellular calciumPyk2Calcineurin
Abstract
Recently, we reported that high extracellular calcium increased receptor activator of nuclear factor-kB ligand(RANKL) expression via p44/42 mitogen-activated protein kinase (p44/42 MAPK) activation in mouse osteoblasts. However, the mechanism for p44/42 MAPK activation by high extracellular calcium is unclear. In this study, we examined the role of intracellular calcium increase in high extracellular calcium-induced RANKL induction and p44/42 MAPK activation. Primary cultured mouse calvarial osteoblasts were used. RANKL expression was highly induced by 10 mM calcium treatment. Ionomycin, a calcium ionophore, also increased RANKL expression and activated p44/42 MAPK. U0126, an inhibitor of MEK1/2, an upstream activator of p44/42 MAPK, blocked the RANKL induction by both high extracellular calcium and ionomycin. High extracellular calcium increased the phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), one of the known upstream regulators of p44/42 MAPK activation....
ISSN
1226-7155
Language
English
URI
http://hdl.handle.net/10371/68595
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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