S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Increace in Intracellular Calcium is Necessary for RANKL Induction by High Extrecellular Calcium
- Kim, Gwan-Shik; Jun, Ji-Hae; Kim, Hyung-Keun; Woo, Kyung-Mi; Baek, Jeong-Hwa
- Issue Date
- Korean Acadamy of Oral Biology
- International Journal of Oral Biology 30:9-15
- Recently, we reported that high extracellular calcium increased receptor activator of nuclear factor-kB ligand(RANKL) expression via p44/42 mitogen-activated protein kinase (p44/42 MAPK) activation in mouse osteoblasts. However, the mechanism for p44/42 MAPK activation by high extracellular calcium is unclear. In this study, we examined the role of intracellular calcium increase in high extracellular calcium-induced RANKL induction and p44/42 MAPK activation. Primary cultured mouse calvarial osteoblasts were used. RANKL expression was highly induced by 10 mM calcium treatment. Ionomycin, a calcium ionophore, also increased RANKL expression and activated p44/42 MAPK. U0126, an inhibitor of MEK1/2, an upstream activator of p44/42 MAPK, blocked the RANKL induction by both high extracellular calcium and ionomycin. High extracellular calcium increased the phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), one of the known upstream regulators of p44/42 MAPK activation....