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Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway

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Authors
Min, Byung-Moo; Chung, Youn Wook; Kim, Tae Soo; Lee, Soo Young; Lee, Seoung Hoon; Choi, Yongwon; Kim, Nacksung; Jeong, Dae-Won; Kim, Ick Young
Issue Date
2006-01
Publisher
Elsevier
Citation
Toxicology Letters 160,143-150
Keywords
SeleniumApoptosisOsteoclastMitochondria
Abstract
The possible effects of sodium selenite on mature osteoclasts were investigated. Incubation of osteoclast-like cells differentiated from RAW 264.7 cells with sodium selenite induced apoptosis as revealed by morphological changes, internucleosomal DNA fragmentation, and activation of caspase-3. Selenite also induced generation of the superoxide anion and reduced the number of free thiol groups in the osteoclast-like cells, suggestive of a shift to a more oxidizing intracellular environment. In addition, selenite induced protein aggregation by thiol cross-linking, loss of the mitochondrial membrane potential, and cytochrome c release in mitochondria isolated from the osteoclast-like cells. Finally, selenite-induced DNA fragmentation in osteoclasts was inhibited both by cyclosporin A, a blocker of the mitochondrial permeability transition pore, and by DEVD-CHO, a cell-permeable inhibitor of caspase-3. These results thus suggest that selenite induces apoptosis mediated by the mitochondrial pathway in mature osteoclasts.
ISSN
0378-4274
Language
English
URI
http://hdl.handle.net/10371/69715
DOI
https://doi.org/10.1016/j.toxlet.2005.06.019
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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